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Freshwater Algae and Cyanobacteria
 

The growth of algae, cyanobacteria and other aquatic plants and animals can affect the quality of water in several ways. They can cause unsightly scums, impart tastes & odours, produce toxins, block filters, increase turbidity and restrict flow in pipes and channels. Cyanobacteria are of most concern, because some species can produce toxins which may have adverse health effects.

The AWQC provides a comprehensive service for the monitoring and assessment of algae and cyanobacteria in source waters for drinking supplies, wastewaters and in fresh and marine waters used for recreation and agricultural/aquaculture use. The Algal Laboratory is NATA accredited for a range of phycological services, including the collection of planktonic and non-planktonic samples, the microscopic identification and enumeration of algal and cyanobacterial taxa and the estimation of their biomass (refer table below).

The majority of samples analysed for algae and cyanobacteria should be received in the laboratory within 24 hours of collection. Customers can choose from a range of turnaround times for reporting of results, including a same day service for samples requiring urgent attention.

Phytoplankton and periphyton are identified from morphological criteria and enumerated by estimation of cell abundance per unit volume. Identification is generally to genus level, but toxin and odour producing taxa of cyanobacteria can be identified to species level. A choice of algal scans (identification only), partial algal counts (selected species), total cyanobacteria and full algal counts are available, depending on the objective of the monitoring program and customer instructions. The uncertainty of measurement in algal enumeration is largely attributable to the clumped distribution of cells in colonies and filaments and sample preparation prior to enumeration can include disaggregation of large aggregates. A measure of cell viability of a cyanobacterial population (eg immediately following algicide treatment) can be determined by microscopic examination of auto-fluorescence.

Total algal biomass is an indicator of trophic status of environmental waters and can be analysed by spectrophotometric determination of chlorophyll pigments (chlorophyll a, chlorophyll b and phaeophytin). Estimates of cyanobacteria biovolume are a useful indicator of potential health risk and can be offered as a measure of species specific biomass, using microscopy and analysis of digitised images.

Certain algae and cyanobacteria produce characteristic odours when present in high numbers and the Biology Unit offers olfactory analysis of hot (60oC) and cold odours, categorised by descriptor and intensity rating as a complementary test to microscopic identification.

Expert advice can be provided on cyanobacteria of concern in Australian freshwaters and the specific algal toxins that they produce. For blooms of unknown toxicity status in potable waters, screening of cyanobacterial samples for toxicity by mouse bioassay is also available. A general screening test for toxicity of blooms in potable water by mouse bioassay can be offered when the class of toxin is unknown.

The Centre has considerable expertise in the investigation and management of water supply problems caused by blooms of nuisance species of algae and cyanobacteria (blue-green algae) in reservoirs, rivers and water treatment plants and the growth of biological fouling organisms in distribution systems, including bryozoa, molluscs and tree roots. Consultancy services can be engaged for specific biological investigations or for the management of cyanobacterial monitoring programs in nominated water bodies.



TEST

METHOD

UNITS

TYPES OF SAMPLES

Algal Scan (Identification)

Microscopic

species level for noxious cyano-bacteria; otherwise genus level

Phytoplankton or benthic algae in freshwaters, marine waters, estuaries, and wastewaters

Algal Identification and Full Enumeration

Microscopic

cells/mL

As above

Algal Identification and Partial Enumeration

Microscopic

cells/mL

As above

Cyanobacterial Identification &Enumeration

Microscopic

cells/mL

As above

Algal Biovolume

Microscopic

mm3/L

Phytoplankton

Chlorophyll - a & b

Spectrophotometric determination

ug/L

Phytoplankton in fresh-waters, marine waters, estuaries and wastewaters

Hot and Cold Odour

Olfactory determination

descriptor and intensity rating

Range of Waters

Algal Toxicity

Mouse Bioassay

mg freeze dried cells/kg mouse wt.

Algal scums



  Algae Fact Sheet

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ABN 69 336 525 019   Last Updated: Thursday, 15 Sep, 2011