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Complete bacterial (bDNA) profiling

It is useful to know the diversity and abundance of aquatic life in water sources, as they can be indicators of a healthy water system and inform catchment management practices.

Conventional methods for detecting bacterial contamination are based on traditional culture techniques which only target specific bacteria, some of which require days of incubation.

Using next-generation sequencing (NGS), we use a simple water sample and determine exactly what bacteria organisms are causing issues.

Bacterial diversity using NGS is becoming a favoured analysis in comparing bacterial profiles using the universal 16S rRNA gene amplicon which has regions that are both highly conserved and highly variable (hypervariable) among bacterial species. The analysis covered nine (V1-V9) of the ION Torrent hypervariable primer regions that are specifically method-validated at the AWQC using our curated and customised databases.

The alterations of the microbiome can define richness, diversity and composition between groups, as well as effects in complex water matrices, bores, wastewater treatment processes and treatment chains.

With three independent bacterial bioinformatics pipeline methods on offer (custom BLAST, QIIME2 and Kraken2), we can customise for any bacterial issue encountered, including complex biofilms.

Results are presented in an accessible, user-friendly report that includes a ranking of the organisms detected in order of relative abundance and percentage of total DNA detected.

Krona plot

Figure. Krona plot

The bacterial DNA data is used to make assessments over time, based on sample baseline data. The bacterial DNA detected in a sample can be monitored to determine if the community structure is shifting to favour different bacterial groups. The bacterial DNA diversity profile represents the current status of the sample and should remain relatively static, depending on the source, volume and (if applicable) disinfection protocols.