Complete bacterial (bDNA) profiling

Our complete bacterial (bDNA) profiling service enables you to determine the diversity and abundance of aquatic life in water sources, knowing they can be indicators of a healthy water system and inform catchment management practices.

Our next-generation sequencing (NGS) methods enables us to use a simple water sample to determine exactly what bacteria organisms are causing issues.

Conventional methods for detecting bacterial contamination are based on traditional culture techniques which only target specific bacteria, some of which require days of incubation.

The bacterial DNA data is used to make assessments over time, based on sample baseline data. The bacterial DNA detected in a sample can be monitored to determine if the community structure is shifting to favour different bacterial groups. The bacterial DNA diversity profile represents the current status of the sample and should remain relatively static, depending on the source, volume and (if applicable) disinfection protocols.

Test results are presented in an accessible, user-friendly report that includes a ranking of the organisms detected in order of relative abundance and percentage of total DNA detected.

DNA Krona plot

Ultimate guide to molecular testing for waterborne pathogens

Discover how the latest DNA testing innovations are key to fully understanding water contamination risks, resolving water quality issues, and preventing waterborne disease outbreaks.

Bacterial diversity using NGS is becoming a favoured analysis in comparing bacterial profiles using the universal 16S rRNA gene amplicon which has regions that are both highly conserved and highly variable (hypervariable) among bacterial species. The analysis covered nine (V1-V9) of the ION Torrent hypervariable primer regions that are specifically method-validated by us using our curated and customised databases.

The alterations of the microbiome can define richness, diversity and composition between groups, as well as effects in complex water matrices, bores, wastewater treatment processes and treatment chains.

With three independent bacterial bioinformatics pipeline methods on offer (custom BLAST, QIIME2 and Kraken2), we can customise for any bacterial issue encountered, including complex biofilms.

Sample container
1.25L DNA free

Label
1.25L - Bacto – ptdna – none - sterile – air gap

Analytes & holding times
All water types
72 hours

Notes
Sampler must follow DNA sampling procedure WI-375